Re-endothelialization of BioVaM using porcine derived endothelial cells

Introduction A persistent problem of the generation of 3D tissues for therapeutic application is efficient nutrient supply and removal of waste products within complex constructs. Depending on the tissue to be reverse engineered, a more or less intense supply of substrates and oxygen must be provided, as diffusion processes are limiting as tissue thickness increases. Thus, the use of decellularized, biological vascularized matrices (BioVaM) with preserved arterial and venous pedicles, aid the generation of perfusable tissue constructs in vitro. To prevent vessel occlusion when implanted, a functional endothelium must line the graft vessel bed. Thus, we describe the re-endothelialization of BioVaM with porcinederived endothelial cells (EC) in vitro.